Antioxidant Properties of Common Edible Plants in Preventing Age-Related Diseases
Main Article Content
Abstract
Background: Oxidative stress contributes to biological processes associated with ageing, while edible plants provide phenolic compounds and other constituents with in-vitro antioxidant activity. Objective: To compare the total phenolic content and antioxidant capacity of ten commonly consumed edible plants obtained from Lahore and determine their relationships across complementary antioxidant assays. Methods: This analytical laboratory study evaluated 30 independently sourced samples representing ten edible plants. Methanolic extracts were assessed for total phenolic content, 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) activity, and ferric-reducing antioxidant power. Plant groups were compared using one-way analysis of variance with Tukey’s post-hoc test. Pearson correlations were calculated from ten plant-level mean values. Results: Amla had the highest total phenolic content (82.45 ± 2.14 mg GAE/g), DPPH inhibition (88.60 ± 1.90%), ABTS activity (91.35 ± 2.20 µmol TE/g), and FRAP value (76.40 ± 1.85 µmol Fe²⁺/g), followed by pomegranate. Tomato had the lowest values across all measurements. Differences among plants were significant for every outcome (all p<0.001). Total phenolic content was strongly correlated with DPPH (r=0.995), ABTS (r=0.995), and FRAP activity (r=0.998; all p<0.001). Conclusion: The selected edible plants differed substantially in in-vitro antioxidant capacity, with amla and pomegranate showing the highest values. These laboratory findings identify locally available sources of antioxidant compounds but do not establish bioavailability or prevention of age-related disease
Article Details
Issue
Section

This work is licensed under a Creative Commons Attribution 4.0 International License.
How to Cite
References
1. Sies H. Oxidative stress: a concept in redox biology and medicine. Redox Biol. 2015;4:180–3. doi:10.1016/j.redox.2015.01.002.
2. Finkel T, Holbrook NJ. Oxidants, oxidative stress and the biology of ageing. Nature. 2000;408(6809):239–47. doi:10.1038/35041687.
3. Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D, et al. Oxidative stress, aging, and diseases. Clin Interv Aging. 2018;13:757–72. doi:10.2147/CIA.S158513.
4. Liu RH. Health-promoting components of fruits and vegetables in the diet. Adv Nutr. 2013;4(3):384S–92S. doi:10.3945/an.112.003517.
5. Iqbal S, Bhanger MI. Effect of season and production location on antioxidant activity of Moringa oleifera leaves grown in Pakistan. J Food Compos Anal. 2006;19(6–7):544–51. doi:10.1016/j.jfca.2005.05.001.
6. Sultana B, Anwar F, Ashraf M. Effect of extraction solvent and technique on the antioxidant activity of selected medicinal plant extracts. Molecules. 2009;14(6):2167–80. doi:10.3390/molecules14062167.
7. Prior RL, Wu X, Schaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem. 2005;53(10):4290–302. doi:10.1021/jf0502698.
8. Aune D, Giovannucci E, Boffetta P, Fadnes LT, Keum N, Norat T, et al. Fruit and vegetable intake and the risk of cardiovascular disease, total cancer and all-cause mortality: a systematic review and dose-response meta-analysis of prospective studies. Int J Epidemiol. 2017;46(3):1029–56. doi:10.1093/ije/dyw319.
9. Miller V, Mente A, Dehghan M, Rangarajan S, Zhang X, Swaminathan S, et al. Fruit, vegetable, and legume intake, and cardiovascular disease and deaths in 18 countries: the Prospective Urban Rural Epidemiology study. Lancet. 2017;390(10107):2037–49. doi:10.1016/S0140-6736(17)32253-5.
10. Wang DD, Li Y, Bhupathiraju SN, Rosner BA, Sun Q, Giovannucci EL, et al. Fruit and vegetable intake and mortality: results from two prospective cohort studies of US men and women and a meta-analysis of 26 cohort studies. Circulation. 2021;143(17):1642–54. doi:10.1161/CIRCULATIONAHA.120.048996.
11. Estruch R, Ros E, Salas-Salvadó J, Covas MI, Corella D, Arós F, et al. Primary prevention of cardiovascular disease with a Mediterranean diet supplemented with extra-virgin olive oil or nuts. N Engl J Med. 2018;378(25). doi:10.1056/NEJMoa1800389.
12. Morris MC, Tangney CC, Wang Y, Sacks FM, Bennett DA, Aggarwal NT. MIND diet associated with reduced incidence of Alzheimer’s disease. Alzheimers Dement. 2015;11(9):1007–14. doi:10.1016/j.jalz.2014.11.009.
13. Valls-Pedret C, Sala-Vila A, Serra-Mir M, Corella D, de la Torre R, Martínez-González MA, et al. Mediterranean diet and age-related cognitive decline: a randomized clinical trial. JAMA Intern Med. 2015;175(7):1094–103. doi:10.1001/jamainternmed.2015.1668.
14. Shishtar E, Rogers GT, Blumberg JB, Au R, Jacques PF. Long-term dietary flavonoid intake and risk of Alzheimer disease and related dementias in the Framingham Offspring Cohort. Am J Clin Nutr. 2020;112(2):343–53. doi:10.1093/ajcn/nqaa079.
15. Zhou Y, Wang J, Cao L, Shi M, Liu H, Zhao Y, et al. Fruit and vegetable consumption and cognitive disorders in older adults: a meta-analysis of observational studies. Front Nutr. 2022;9:871061. doi:10.3389/fnut.2022.871061.
16. Fangfang H, Qiong W, Shuai Z, Xiao H, Jingya Z, Guodong S, et al. Vegetable and fruit intake, its patterns, and cognitive function: cross-sectional findings among older adults in Anhui, China. J Nutr Health Aging. 2022;26(5):529–36. doi:10.1007/s12603-022-1791-y.
17. Yeung SSY, Kwok T, Woo J. Higher fruit and vegetable variety associated with lower risk of cognitive impairment in Chinese community-dwelling older men: a 4-year cohort study. Eur J Nutr. 2022;61(4):1791–9. doi:10.1007/s00394-021-02774-y.
18. Huang L, Zhao C, Gao M, Tao Y, Chen X, Chen H, et al. Associations of vegetable and fruit intake with cognitive function and its decline: two longitudinal studies. J Nutr Health Aging. 2024;28(6):100223. doi:10.1016/j.jnha.2024.100223.
19. Sultana B, Anwar F, Przybylski R. Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam. trees. Food Chem. 2007;104(3):1106–14. doi:10.1016/j.foodchem.2007.01.019.
20. Shan S, Huang X, Shah MH, Abbasi AM. Evaluation of polyphenolics content and antioxidant activity in edible wild fruits. Biomed Res Int. 2019;2019:1381989. doi:10.1155/2019/1381989.
21. Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol. 1995;28(1):25–30. doi:10.1016/S0023-6438(95)80008-5.
22. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26(9–10):1231–7. doi:10.1016/S0891-5849(98)00315-3.
23. Benzie IFF, Strain JJ. The ferric reducing ability of plasma as a measure of antioxidant power: the FRAP assay. Anal Biochem. 1996;239(1):70–6. doi:10.1006/abio.1996.0292.
24. Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of the Folin–Ciocalteu reagent. Methods Enzymol. 1999;299:152–78. doi:10.1016/S0076-6879(99)99017-1.