Establishment of Reference Ranges for CBC Parameters in Adult Pakistani Population
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Background: Complete blood count interpretation depends on reference intervals that may vary by sex, ethnicity, geography, altitude, nutrition, and analytical methods. Reliance on international reference ranges may be inappropriate for local Pakistani populations, particularly in high-altitude regions. Objective: To establish sex-specific reference intervals for selected complete blood count parameters among apparently healthy adults from Quetta and surrounding areas. Methods: This cross-sectional observational study was conducted in the Department of Pathology, Combined Military Hospital, Quetta, from December 2020 to November 2021. A total of 300 apparently healthy adults aged 20–55 years were recruited, including 150 males and 150 females. Venous blood samples were collected in EDTA tubes and analyzed using a SYSMEX XP-100 automated hematology analyzer. Hemoglobin, total leukocyte count, platelet count, red blood cell count, mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration were recorded. Reference intervals were calculated using the 2.5th and 97.5th percentiles. Results: Males showed higher mean hemoglobin, red blood cell count, mean corpuscular volume, and mean corpuscular hemoglobin, while females showed higher total leukocyte count, platelet count, and mean corpuscular hemoglobin concentration. Male and female hemoglobin reference intervals were 12.6–15.9 g/dL and 10.3–13.1 g/dL, respectively. Conclusion: Sex-specific CBC reference intervals differed from commonly used international ranges, supporting locally derived interpretation in this population
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